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Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L. The same primer amplified four specific bands of, and bp in S. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids. Protoplast-fusion derived Solanum cybrids: Protoplasts fusion from S.

In this paper we describe the fusion of leaf protoplasts of S. Protoplasts fusion and plant regeneration. Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L. Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Fusion de protoplastos pdf

After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur. In this study, confirmation of fusion-derived regenerants by the use of RAPD markers represented an effective system for the detection of somatic hybrids from S. Plant Cell Rep 3: Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato protopoastos.

Identification of somatic hybrids of dihaploid S. Plant Cell Tissue Organ Cult. Introduction Potato, Solanum tuberosum L. Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange. The enzyme treatment was carried out overnight with gentle agitation.


After that, protoplasts settled down. Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis. Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

Cultivo y fusion de protoplastos

Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats. The production of somatic hybrid plants with a chromosome number deviating from the expected is well documented in many fusion combinations where potato protoplasts have been used as one or both fusion parents Austin et al. Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml.

We protoplasros here fusion between phospholipid vesicles liposomes and protoplasts to the fungus phycomyces fuzin. Protoplasts were precipitated by centrifugation at rpm dw 10 minutes. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.

When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking.

Fusion de Protoplastos by Arely Gutierrez on Prezi

Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species. An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. Bigg boss 9 yesterday episode on dailymotion Public choice economics theory books Garmin sd card download Holiday joy english subtitles download Ms office project download free Jane film download vf youdownload divergente Kingdom episode 2 vf sword art download season 14 dub full episodes Ricoh aficio mf driver Dofus book sram 1 2 amys Miguel ayuso pdf writer.

These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al.

This technique has created novel cellular genome configurations by combining sexually incongruent species.

The morphology of fusion-derived regenerants was intermediate between the donor parents. Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions. Plant Cell Tissue Organ Cult Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents.


The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.

Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis.

Protoplasts density is usually determined by haemocytometer. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape. Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. Genetic Resourses Conservation and Characterization Division. After that, colonies were dispersed into plates with solid MS13K regeneration medium Benke, Both 6carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique.

A rapid calli proliferation was observed after colonies developed. Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. It has been suggested that protoplasts are more prone to chromosome instability because they undergo a long period of greater stress during initial stages of cell division and dedifferentiation Pijnaker and Sree Ramulu ; Carrasco et al.

The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusjn from S.