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Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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The microbial utilization of such carbon-rich, but nitrogen-poor substrates necessitates the availability of nitrogenous compounds for the synthesis of cell materials such as proteins and nucleic acids. Some bacteria contain catabolic plasmids that can transform hydrocarbon pollutant into simple organic molecule; plasmid genes encode for enzymes for ring cleavage and oxidation reactions.

Sorkhoh1 and Samir S. Crude oils are composed of an array of chemical compounds, minor constituents, and trace metals. The probable reason why MCC was more effective than the pure isolates might be as a result of the synergistic effect of enzymatic reactions from various bacteria isolates on the refinery pollutant or the composite effect of enzymes of the five isolates acting more on metabolites than enzymes of individual isolates. The growth curves in Fig. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content.

Previous studies, mainly from our laboratory, described the combined potential of certain bacterial species for hydrocarbon utilization and nitrogen fixation.

Potential Hydrocarbonoclastic Bacteria

A cell suspension was prepared for each organism by mixing a loopful from a pure h culture in 5 mL of the mineral nitrogen-free medium in a Hungate tube with a tight rubber cap. They are rarely present in unpolluted areas! Atlas RM, Bartha R. Mechanisms and occurrence of microbial oxidation of long-chain alkanes. Various products and microorganisms are useful in these applications and each will yield different results.


Soil bacteria mentioned above gacteria also found in freshwater as they are carried by rainwater runoff, water drainage or via discharge of effluents. Results and Discussion Growth on nitrogen-free medium and possession of nifH genes Eighty-two out of the hydrocarbonoclastic bacterial species tested were capable of growing on the NaNO 3 -free mineral medium with oil as the sole source of carbon and energy.

These are microbial-synthesized surface-active substances that allow for more efficient microbial biodegradation of hydrocarbons in bioremediation processes. Nitrogen gas was needed by hydrocarbonoclastic bacteria for growth and oil consumption in the absence of NaNO 3 The growth curves in Fig. In more complicated cases, various methods of adding bacterla, air, or exogenous microorganisms to the contaminated site can be applied. Support Center Support Center.

Petroleum microbiology – Wikipedia

Journal List Microbes Environ v. The results obtained clearly demonstrated that oil consumption was markedly effective in the NaNO 3 -containing medium irrespective of whether nitrogen gas was present in the top space. Proteus vulgarisKlebsiella pneumoniae, Serretia marcescens and Streptomyces sp.

Similarly, biosurfactants produced by HCB disperse hydrophobic hydrocarbon molecules thereby increasing their surface area to enhance the growth bacteriaa efficiency of oil utilization. Comments on this article: The gene amplicons were subsequently separated and detected by gel electrophoresis.

Please introduce links to this page from related articles ; try the Find link tool for suggestions. Larger oil droplets cannot be taken up by bacterial cells. Interest in this field is growing due to the increasing role of bioremediation in oil spill cleanup.

Therefore, we examined the most frequently detected species in desert soil and seawater in Kuwait. Eukaryotic organisms appeared later in the course of evolution. Microbial biosensors identify and quantify target compounds of hydrocarbonoclqstic through interactions with the microbes.


One hundred-milliliter aliquots of the mineral medium 24 amended with 0.

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Therefore, these bacteria are useful for the cost-effective, environmentally friendly bioremediation of hydrocarbon contaminants. This result corroborates with the report of Kaplan bacteroa Kitts Collection of Sample 2.

Numerous genera of bacteria are known to be good hydrocarbon degraders and most of them belong to the genera Pseudomonas, Sphingomonas, Aeromonas, Alcaligenes, Staphylococcus, Lacticobacillus, Acinetobacter, Arthrobacter, Brevibacterium, Xanthomonas, Mycobacterium, Micrococcus, Neisseria, Enterobacter, Rhodococcus and Bacillus species Plaza et al.

Frontiers in Environmental Microbiology. The results obtained is relatively lower than that of Abdullahi et al. Hydrocarbonoclastic potential of HCB in freshwater is dependent on emulsification or surfactant activity of strains, pH, concentration of contaminant in that habitat. In the present study, we demonstrated that hydrocarbonoclastic bacteria isolated from all over Kuwait exhibited diazotrophic potential, as well as actual bactwria activity.

When further supplied with sufficient limiting nutrients such as nitrogen and phosphor, it grows and produces surfactant glucolipids hydrkcarbonoclastic help reduce surface water tension and enhance hydrocarbon uptake.

Bioremediation, Hydrocarbonoclastic, Bacteria, Refinery Effluent. PAHs present as natural constituents in fossils fuels are formed during the hydrocarbonpclastic combustion of organic material and are therefore presents in relatively high concentrations in products of fossil fuels refining Wang et al. They have been exploited for their biodegradation potential and have been used successfully for cleanup of oil contaminated soils and aquatic systems.

Rehm HJ, Reiff I. Biofilm comprising phototrophic, diazotrophic, and hydrocarbon-utilizing bacteria: Indigenous species bloom after the hydrocarbon pollution.